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<p>37°C에서 배양했을 때, Col I은 기질 응고 과정에서 BsM 조각을 둘러싸는 연속적인 섬유 네트워크를 형성했습니다. 60% vol/vol BsM(조각)-1.5 mg/mL Col I의 저장 탄성률(약 160 Pa)은 조각이 없는 60% vol/vol BsM-1.5 mg/mL Col I(약 470 Pa)보다 현저히 낮았지만, 50% vol/vol BsM 단독(약 10 Pa)보다는 여전히 한 자릿수 높았습니다( 그림 1G 및 S2D ). 피코시리우스 레드 염색 결과, BsM(조각)-Col I 기질에서 Col I 섬유 다발이 조각이 없는 기질에 비해 질적으로 더 길다는 것을 알 수 있었으며( 그림 1H ), 이는 가교 밀도 및 강성 감소와 관련이 있었습니다 .37 마지막으로, BsM 조각화가 분화 과정에서 UB 표현형에 부정적인 영향을 미치지 않는다는 것을 확인했습니다. 위상 이미징 결과, 50% vol/vol BsM, 60% vol/vol BsM-1.5 mg/mL Col I, 그리고 60% vol/vol BsM(frag.)-1.5 mg/mL Col I 매트릭스에서 배양된 UB 오가노이드의 크기와 형태가 유사한 것으로 나타났습니다( 그림 1 I). UB, CD 및 오프타겟 마커를 포함한 172개 유전자에 대한 전사체 분석 결과, 각 매트릭스에서 배양된 오가노이드 간에 유의미한 차이가 없었습니다( 그림 S3 ). 따라서 60% vol/vol BsM(frag.)-1.5 mg/mL Col I 매트릭스가 단층 형성, 분지 형태 발생 및 내장형 바이오프린팅에 적합한 것으로 판단되었습니다. 이후 모든 연구에서는 이 매트릭스를 BsM(frag.)-Col I로 명명하여 사용했습니다. 3D UB 세관 제작</p> <p><a class="seoquake-nofollow" href="https://bjlol.clickn.co.kr/" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"><span style="color:rgb(255,255,255);font-family:LexiSaebomR;line-height:2;letter-spacing:1px;text-decoration:none;">롤토토</span></a> <a class="seoquake-nofollow" href="https://zzgtv.com/" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"> <span style="color:rgb(255,255,255);font-family:LexiSaebomR;line-height:2;letter-spacing:1px;text-decoration:none;">짱구티비</span></a> <a class="seoquake-nofollow" href="https://sports.clickn.co.kr/" rel="nofollow noreferrer noopener" 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