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<p>표면 패시베이션 패턴이 없는 영역에 혈소판이 부착되는 것을 방지하기 위해, 미세 패턴이 있는 유리 바닥 접시는 10 mM HEPES(#H3537; Sigma-Aldrich)에 0.01 mg/mL의 폴리(L-리신)(20)-g[3.5]-폴리(에틸렌 글리콜)(2)(SuSoS, Duebendorf, Switzerland)을 첨가하여 37°C에서 1~3시간 동안 코팅하여 패시베이션 처리하였다. 코팅된 미세 패턴 접시는 고성능 액체 크로마토그래피용 증류수(#10449380; Fisher Scientific, Schwerte, Germany)로 세 번 세척하였다. 각 측정일마다 코팅은 새로 준비하였다. 혈소판 분리 모든 절차는 기관 윤리위원회(273/2018BO2)의 승인을 받았으며 헬싱키 선언을 준수했습니다. 연구에 참여한 모든 대상자로부터 사전 동의를 받았습니다. 혈액은 건강한 지원자로부터 산성 시트르산 포도당 용액(ACD, #C3821, Sigma-Aldrich)에 담아 채취했습니다(ACD/혈액량 비율 1:4). 혈액은 사용 전까지 37°C에서 저속으로 지속적으로 회전시키면서 보관했습니다. 혈소판 풍부 혈장(PRP)은 200 × g 에서 20분간 원심분리하여 얻었습니다. 세척된 혈소판을 얻기 위해, 1.5 mL의 PRP를 4.5 mL의 Tyrode 완충액(137 mM NaCl, 3 mM KCl, 1 mM MgCl₂ , 0.4 mM NaH₂PO₄ , 12 mM NaHCO₃ , 5.5 mM D-포도당, 4 mM HEPES, 1 g/L 소 혈청 알부민) (pH 6.5)에 첨가한 후, 880 × g 에서 10분간 두 번째 원심분리를 수행하였다. 이렇게 얻은 혈소판 침전물을 Tyrode 완충액(pH 7.4)에 재현탁시켰다. 이렇게 얻은 혈소판 현탁액을 실험에 즉시 사용하였다. <a class="seoquake-nofollow" href="https://blacktv24.com/" rel="nofollow noreferrer noopener" 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